Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli.
نویسندگان
چکیده
To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 21 1 شماره
صفحات -
تاریخ انتشار 2011